STEMCCA Vector Advantages: Efficient: uses a single vector with four transcription factors rather than … Recommended Usage. Successful generation of iPS cells from human foreskin fibroblasts after infection with single-vector Human STEMCCA Cre-Excisable Lentivirus (Cat. Not for use in diagnostic procedures. The CytoTune™-iPS 2.0 Sendai Reprogramming Kit typically exhibits greater than 2-fold efficiency over the original kit, for both fibroblast and blood reprogramming. All components of the kit are sterile and have tested negative for Mycoplasma. 8. 6556 0 obj <>/Filter/FlateDecode/ID[<4CECC13F6160584A913DDE182A90E957><8E2D541F017DB34B8CAD0490E55E2082>]/Index[6531 47]/Info 6530 0 R/Length 110/Prev 2245953/Root 6532 0 R/Size 6578/Type/XRef/W[1 2 1]>>stream Resulting passage 3 human iPS cells exhibit high alkaline phosphatase activity (A), Oct-4 expression (B), SSEA-3 expression (C), and TRA-1-60 expression (D). h��UmLSg>�-\��-���Q�sݤZ?��_�qJ\\��ִx���,�����sq�:*�� e�֏E��d��KZ!�,Q�f?���ع�-`�,��5���� Nuclei are stained with DAPI (blue). h�bbd``b`� �)@��H0ǃ�K"�U"V��+ %�A+�C��$�A�8 ����z@��@�/ Like the CytoTune-iPS 2.0 Sendai reprogramming kit, this kit uses Sendai particles to deliver Yamanaka factors that are critical for efficient generation of induced pluripotent stem cells (iPSCs). 0 �jIT NA� The CytoTune™-iPS Reprogramming Kit provides a 100-fold increase in efficiency over standard methods to generate iPSCs, allowing for reprogramming efficiencies between 0.01% to 1%. Efficiencies greater than 1% have been obtained with both fibroblasts and blood cells. Store at -5 to -20°C. Regardless of where you are in the process, we have reprogramming tools to support you from research through commercialization.Learn how to achieve successful reprogramming while reducing hands-on time.Discover how to generate iPSCs directly from blood cells without lengthy culture steps.Find out how Sendai works in multiple starting cell types.For Research Use Only. Simplicon™ RNA Reprogramming Kit (OKSG) EMD Millipore’s Simplicon™ RNA Reprogramming Kit is a safe and efficient method to generate integration free, virus-free human iPS cell using a single transfection step of self-replicating synthetic RNA for Oct4, Klf4, Sox2 and Glis1. One kit of four tubes is enough for 2 wells of a 6-well plate (5 x 105cells/well) at MOI = 3. No. SCR545), as indicated by expression of characteristic pluripotency markers. Human iPS Cell Reprogramming Episomal Kit is a cost-effective plasimd mix of multiple episomal vectors which encode four reprogramming factors.
The CytoTuneiPS Reprogramming Kit contains four SeV™- -based reprogramming vectors, each capable of expressing one of the four Yamanaka factors (i.e., Oct4, Sox2, Klf4, and c- Myc) and are optimized for generating iPSCs from human somatic cells. For use with associated StemRNA 3 rd Gen Reprogramming Kit protocols for generation of iPS cells. How many cells can I reprogram with one set of viral parti- cles? %PDF-1.6 %���� 6577 0 obj <>stream Not for use in diagnostic procedures. Other reprogramming methods, such as lentivirus and retrovirus, contain transgenes that can integrate into the host genome, potentially disrupting the genome or causing unpredictable results. The Epi5™ Episomal iPSC Reprogramming Kit provides an easy-to-use, highly efficient set of 5 episomal vectors designed by Dr. Okita in the laboratory of Professor Yamanaka at the Center for iPS Cell Research and Application (CiRA), Kyoto University. Derive cell lines from diverse genetic backgrounds directly starting from a myriad of cell types, including PBMCs and T-cells. Retrovirus iPSC Reprogramming Kit: #RF101 Product Description Human induced pluripotent stem cells (iPSCs) can be derived from somatic cells through a reprogramming process driven by ectopic expression of a defined set of reprogramming factors: Oct4, Sox2, Klf4 and c-Myc.
Generation of induced pluripotent stem cells (iPSCs) from somatic cells is no longer an art; it’s predictable science with CytoTune-iPS Sendai reprogramming kits. Use the links below to find out how the Invitrogen CytoTune-iPS 2.0 Sendai Reprogramming Kit can revolutionize your cell reprogramming. The reprogramming vectors in this kit have been engineered to increase biological and environmental safety (see Safety features of the system, page 8). Complete reprogramming of iPS cell colonies is confirmed by expression of pluripotency markers and appropriate colony morphology.
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-iPS 2.0 Sendai Reprogramming Kit contains three SeV-based reprogramming vectors, and are optimized for generating iPSCs from human somatic cells. All components of the kit are sterile and have tested negative for Mycoplasma spp. Kit Contents Kit Contents The STEMCCA vector is comprised of the transcription factors Oct-4, Klf4, SOX-2, and c-Myc (OKSM), separated by the self-cleaving 2A peptide and IRES sequences driven by the EF-1α constitutive promoter %%EOF STEMCCA™ Lentivirus Reprogramming Kits Figure: Successful generation of iPS cells from human foreskin fibroblasts after infection with single-vector Human STEMCCA Cre-Excisable Lentivirus (Cat.
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